US Series-A VCs

Today, we selected 20 active US Series-A VCs from our database, investing in startups.  

Our team reviewed, checked, and enriched the list with the necessary data.

(The first 10 are free for all subscribers, the last 10 are open to our premium subscribers.)

Here goes:

• 1Flourish Capital — Mountain View-based 1Flourish Capital is a faith-driven venture firm that invests in A-round companies. The firm seeks and supports entrepreneurs who value principled leadership and strong company culture as essential drivers of high-performance businesses. As a venture capital investor, 1Flourish emphasizes the integration of values and long-term business success in its portfolio approach.

• 2.0 Ventures — Salt Lake City-based 2.0 Ventures partners with visionary consumer and technology startups at the A-round stage. As a venture capital firm, it provides investment and operational support to help founders overcome growth challenges, optimize for financing or exits, and sustain profitability. The firm’s philosophy emphasizes flexibility and open-minded collaboration between investors and entrepreneurs to drive long-term success.

• 3Lines — Greenwood Village-based 3Lines is a venture capital firm investing in A-round disruptive technology companies. The firm focuses on themes such as the future of work, enterprise, and industry, backing founders who are driving structural change in these sectors. By supporting companies at inflection points, 3Lines aims to fuel scalable innovation with broad market impact.

• 5AM Ventures — Menlo Park-based 5AM Ventures is a venture capital firm specializing in biotechnology. Focused on A-round and early-stage life sciences companies, it provides capital to entrepreneurs working on transformative healthcare solutions. The firm’s strategy centers on building and financing businesses that can advance innovation and improve patient outcomes through scientific breakthroughs.

• 7wire Ventures — Chicago-based 7wire Ventures is a venture capital firm investing in A-round healthcare startups. The firm backs early and growth-stage companies developing digital health, healthcare IT, and mobile health solutions. Its mission is to transform patient care through technology-enabled approaches that improve access, efficiency, and health outcomes in the healthcare ecosystem.

• 8-Bit Capital — San Francisco-based 8-Bit Capital is a venture capital firm that invests in early-stage software startups at the A-round stage. With a focus on cybersecurity, fintech, AI, enterprise, cloud, and social technologies, it backs companies that connect people and businesses in innovative ways. The firm’s sector breadth reflects its emphasis on scalable, technology-driven solutions.

• 9Yards Capital — San Francisco-based 9Yards Capital is a global venture capital firm investing at the A-round and growth stage. Its portfolio spans consumer products, services, fintech, and transportation, with a focus on tech-enabled businesses. By backing companies with global potential, 9Yards positions itself as a long-term partner for founders scaling complex industries.

• 12/12 Ventures — Sausalito-based 12/12 Ventures is a venture capital firm targeting A-round opportunities in the cannabis industry. The firm focuses on supporting innovative entrepreneurs in this emerging and rapidly evolving sector. By providing capital and strategic guidance, it helps businesses scale responsibly within a highly regulated market environment.

• 20/20 HealthCare Partners — Auburndale-based 20/20 HealthCare Partners is a venture capital group that invests at the A-round in healthcare and life sciences. With a global perspective, the firm emphasizes early-stage technology ventures in the health sector. Its strategy is to back founders developing new tools and treatments that reshape how healthcare is delivered and advanced.

• 137 Ventures — San Francisco-based 137 Ventures is a growth-stage venture capital firm investing in A-round technology companies. It specializes in providing liquidity solutions tailored to founders, employees, and investors of high-growth private firms. By offering flexible capital, 137 Ventures supports entrepreneurs while enabling continued growth and value creation.

That’s all for today. Have a great day!

Tzakhi

Founder of InvestorsLists.VC and Meet.Capital 

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